ABSTRACT
This study proposes an image segmentation method based on bottleneck detection and watershed algorithm to solve the problem of overlapping cervical cell image. First, we use polygon approximation to get all feature points on the cell contour and then use bottleneck detection and ellipse fitting to obtain the correct split point pairs. Therefore, the approximate range of the overlapping region was determined. The watershed algorithm was used to obtain the internal boundary information for the gradient image of the region. Finally, the segmentation results of the overlapped cells were obtained by superimposing with the outer contour. The experimental results show that this algorithm can segment the contour of a single cell from the overlapping cervical cell images with good accuracy and integrity. The segmentation result is close to that of doctors' manual marking, and the segmentation result is better than other existing algorithms.
Subject(s)
Female , Humans , Algorithms , Cervix Uteri/cytology , Image Processing, Computer-AssistedABSTRACT
Objective To investigate the morbidity of rapid eye movement sleep behavior disorder(RBD) in early Parkinson disease (PD)and its related factors. Methods One hundred twenty-five early PD patients were divided into RBD group (n=51)and non-RBD group (n=74),according to the complicated with RBD.We collected the clinical data and used univariate and multivariate logistic regression methods to analyze the risk factors for rapid eye movement sleep behavior disorder in early Parkinson disease. Results The incidence of RBD is 40.80% in early PD patients.Univariate analysis showed that the age, the motor phenotype (akinetic-rigid-type), modified Hoehn-Yahr(H-Y)grade, freezing, constipation and restless leg syndrome (RLS)were significantly higher in RBD group than in non-RBD group (P<0.05). Multivariate logistic regression revealed that constipation ( P=0.001,95% CI:1.980~12.253,OR=12.912)and RLS (P=0.014, 95% CI: 1.322~12.015, OR=6.378 ) were independent influencing factors for RBD in early PD patients. Conclusion Early PD patients with constipation or RLS are prone to RBD.
ABSTRACT
Bone marrow stromal cells are a class of multipotent stem cells.They have self-renewal and multilineage differentiation potential and provide a basis for cell and gene therapy in a variety of diseases.Many experimental studies shown that bone marrow stromal cell transplantation has a significant therapeutic effect for cerebral infarction.This article reviews the method and effect of bone marrow stromal cells in treatment of cerebral infarction in recent years.
ABSTRACT
Bone marrow stromal cells are a kind of non-hematopoietic stem cells in the bone marrow.They possess self-renewing capability and multilineage differentiation potential.They are the ideal cell source for cell transplantation in the treatment of various diseases.A large number of animal model experiments shown that BMSC transplantation can promote brain structure and functional recovery after cerebral ischemia through a variety of mechanisms and provide new ideas and methods for the treatment of cerebrovascular diseases.This article reviews the mechanisms of BMSCs in the treatment of ischemic stroke in recent years.